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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in several tumors, which leads to chromosome instability, and therefore might lead to tumorigenesis. This protein was located to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a critical position in advertising mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been described. Two more splice variants have been documented but specific nucleotide sequence information and facts is just not nonetheless accessible.
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Blocking peptides are peptides that bind exclusively for the target antibody and block antibody binding. These peptide normally consists of the epitope identified via the antibody.
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This tutorial outlines further more achievable optimization of mobile and immunoassay parameters to make certain the best possible effects are...
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This guideline outlines more feasible optimization of mobile and immunoassay parameters to be sure the absolute best benefits are...
Antibodies sure to the blocking peptide not bind to the epitope within the concentrate on protein. By evaluating the staining with the blocked antibody compared to the antibody by itself, one can see which staining is specific.
Concentrate on Data Cyclin E belongs to the extremely conserved cyclin loved ones, whose associates are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which add on the temporal coordination of each mitotic celebration. Cyclin E types a posh with and features to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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